prikaz prve stranice dokumenta Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3
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Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3
International Journal of Molecular Sciences, 24 (2023), 20; 15269. https://doi.org/10.3390/ijms242015269
Vasiljević, Tea; Tarle, Marko; Hat, Koraljka; Lukšić, Ivica; Mikulandra, Martina; Busson, Pierre; Matijević Glavan, Tanja

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Vasiljević, T., Tarle, M., Hat, K., Lukšić, I., Mikulandra, M., Busson, P. i Matijević Glavan, T. (2023). Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3. International Journal of Molecular Sciences, 24. (20). doi: 10.3390/ijms242015269

Vasiljević, Tea, et al. "Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3." International Journal of Molecular Sciences, vol. 24, br. 20, 2023. https://doi.org/10.3390/ijms242015269

Vasiljević, Tea, Marko Tarle, Koraljka Hat, Ivica Lukšić, Martina Mikulandra, Pierre Busson i Tanja Matijević Glavan. "Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3." International Journal of Molecular Sciences 24, br. 20 (2023). https://doi.org/10.3390/ijms242015269

Vasiljević, T., et al. (2023) 'Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3', International Journal of Molecular Sciences, 24(20). doi: 10.3390/ijms242015269

Vasiljević T, Tarle M, Hat K, Lukšić I, Mikulandra M, Busson P, i sur.. Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3. International Journal of Molecular Sciences [Internet]. 17.10.2023. [pristupljeno 03.07.2024.];24(20). doi: 10.3390/ijms242015269

T. Vasiljević, et al., "Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3", International Journal of Molecular Sciences, vol. 24, br. 20, Listopad 2023. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:127:012989. [Citirano: 03.07.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:127:012989
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Naslov (engleski)Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3
AutorTea Vasiljević
AutorMarko Tarle
AutorKoraljka Hat
AutorIvica Lukšić
AutorMartina Mikulandra
AutorPierre Busson
AutorTanja Matijević Glavan
Autorova ustanovaInstitut Ruđer Bošković
Autorova ustanova(Klinička bolnica Dubrava)
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Autorova ustanovaSveučilište u Zagrebu
Stomatološki fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Dentalna medicina
Sažetak (engleski)
Tumor necrosis is a recurrent characteristic of head and neck squamous cell carcinomas (HNSCCs). There is a need for more investigations on the influence of biomolecules released by these necrotic foci in the HNSCC tumor microenvironment. It is suspected that a fraction of the biomolecules released by necrotic cells are damage-associated molecular patterns (DAMPs), which are known to be natural endogenous ligands of Toll-like receptors (TLRs), including, among others, proteins and nucleic acids. However, there has been no direct demonstration that biomolecules released by HNSCC necrotic cells can activate TLRs. Our aim was to investigate whether some of these molecules could behave as agonists of the TLR3, either in vitro or in vivo. We chose a functional approach based on reporter cell exhibiting artificial TLR3 expression and downstream release of secreted alkaline phosphatase. The production of biomolecules activating TLR3 was first investigated in vitro using three HNSCC cell lines subjected to various pronecrotic stimuli (external irradiation, serum starvation, hypoxia and oxidative stress). TLR3 agonists were also investigated in necrotic tumor fluids from five oral cancer patients and three mouse tumor grafts. The release of biomolecules activating TLR3 was demonstrated for all three HNSCC cell lines. External irradiation was the most consistently efficient stimulus, and corresponding TLR3 agonists were conveyed in extracellular vesicles. TLR3-stimulating activity was detected in the fluids from all five patients and three mouse tumor grafts. In most cases, this activity was greatly reduced by RNAse pretreatment or TLR3 blocking antibodies. Our data indicate that TLR3 agonists are consistently present in necrotic fluids from HNSCC cells and mainly made of dsRNA fragments. These endogenous agonists may induce TLR3, which might lead to a protumorigenic effect. Regarding methodological aspects, our study demonstrates that direct investigations—including functional testing—can be performed on necrotic fluids from patient tumors.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaInternational Journal of Molecular Sciences
Brojčani podacivol. 24, br. 20, 15269
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms242015269
URN:NBNurn:nbn:hr:127:012989
Datum objave publikacije2023-10-17
URL dokumentahttps://www.mdpi.com/1422-0067/24/20/15269
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2024-07-03 08:34:21