prikaz prve stranice dokumenta Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3
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Scientific paper - Original scientific paper
Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3
International Journal of Molecular Sciences, 24 (2023), 20; 15269. https://doi.org/10.3390/ijms242015269
Vasiljević, Tea; Tarle, Marko; Hat, Koraljka; Lukšić, Ivica; Mikulandra, Martina; Busson, Pierre; Matijević Glavan, Tanja

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Vasiljević, T., Tarle, M., Hat, K., Lukšić, I., Mikulandra, M., Busson, P. & Matijević Glavan, T. (2023). Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3. International Journal of Molecular Sciences, 24. (20). doi: 10.3390/ijms242015269

Vasiljević, Tea, et al. "Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3." International Journal of Molecular Sciences, vol. 24, no. 20, 2023. https://doi.org/10.3390/ijms242015269

Vasiljević, Tea, Marko Tarle, Koraljka Hat, Ivica Lukšić, Martina Mikulandra, Pierre Busson and Tanja Matijević Glavan. "Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3." International Journal of Molecular Sciences 24, no. 20 (2023). https://doi.org/10.3390/ijms242015269

Vasiljević, T., et al. (2023) 'Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3', International Journal of Molecular Sciences, 24(20). doi: 10.3390/ijms242015269

Vasiljević T, Tarle M, Hat K, Lukšić I, Mikulandra M, Busson P, and sur.. Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3. International Journal of Molecular Sciences [Internet]. 2023 October 17 [cited 2024 July 03];24(20). doi: 10.3390/ijms242015269

T. Vasiljević, et al., "Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3", International Journal of Molecular Sciences, vol. 24, no. 20, October 2023. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:127:012989. [Accessed: 03 July 2024]

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Metadata
Title (english)Necrotic Cells from Head and Neck Carcinomas Release Biomolecules That Are Activating Toll-like Receptor 3
AuthorTea Vasiljević
AuthorMarko Tarle
AuthorKoraljka Hat
AuthorIvica Lukšić
AuthorMartina Mikulandra
AuthorPierre Busson
AuthorTanja Matijević Glavan
Author's institutionRuđer Bošković Institute
Author's institution(Dubrava University Hospital)
Author's institutionUniversity of Zagreb
School of Medicine
Author's institutionUniversity of Zagreb
School of Dental Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Dental Medicine
Abstract (english)
Tumor necrosis is a recurrent characteristic of head and neck squamous cell carcinomas (HNSCCs). There is a need for more investigations on the influence of biomolecules released by these necrotic foci in the HNSCC tumor microenvironment. It is suspected that a fraction of the biomolecules released by necrotic cells are damage-associated molecular patterns (DAMPs), which are known to be natural endogenous ligands of Toll-like receptors (TLRs), including, among others, proteins and nucleic acids. However, there has been no direct demonstration that biomolecules released by HNSCC necrotic cells can activate TLRs. Our aim was to investigate whether some of these molecules could behave as agonists of the TLR3, either in vitro or in vivo. We chose a functional approach based on reporter cell exhibiting artificial TLR3 expression and downstream release of secreted alkaline phosphatase. The production of biomolecules activating TLR3 was first investigated in vitro using three HNSCC cell lines subjected to various pronecrotic stimuli (external irradiation, serum starvation, hypoxia and oxidative stress). TLR3 agonists were also investigated in necrotic tumor fluids from five oral cancer patients and three mouse tumor grafts. The release of biomolecules activating TLR3 was demonstrated for all three HNSCC cell lines. External irradiation was the most consistently efficient stimulus, and corresponding TLR3 agonists were conveyed in extracellular vesicles. TLR3-stimulating activity was detected in the fluids from all five patients and three mouse tumor grafts. In most cases, this activity was greatly reduced by RNAse pretreatment or TLR3 blocking antibodies. Our data indicate that TLR3 agonists are consistently present in necrotic fluids from HNSCC cells and mainly made of dsRNA fragments. These endogenous agonists may induce TLR3, which might lead to a protumorigenic effect. Regarding methodological aspects, our study demonstrates that direct investigations—including functional testing—can be performed on necrotic fluids from patient tumors.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 24, no. 20, 15269
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms242015269
URN:NBNurn:nbn:hr:127:012989
Publication2023-10-17
Document URLhttps://www.mdpi.com/1422-0067/24/20/15269
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-07-03 08:34:21