prikaz prve stranice dokumenta Fourier Transform Infrared Spectroscopy Reveals Molecular Changes in Blood Vessels of Rats Treated with Pentadecapeptide BPC 157
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Fourier Transform Infrared Spectroscopy Reveals Molecular Changes in Blood Vessels of Rats Treated with Pentadecapeptide BPC 157
Biomedicines, 10 (2022), 12; 3130. https://doi.org/10.3390/biomedicines10123130
Gamulin, Ozren; Oroz, Katarina; Coric, Luka; Krajacic, Maria; Skrabic, Marko; Dretar, Vilim; Strbe, Sanja; Talapko, Jasminka; Juzbasic, Martina; Krezic, Ivan; Lozic, Marin; Stambolija, Vasilije; Zizek, Helena; Jurca, Ivana; Jurjevic, Ivana; Blagaic, Alenka Boban; Skrtic, Anita; Seiwerth, Sven; Sikiric, Predrag Više autora...

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Gamulin, O., Oroz, K., Coric, L., Krajacic, M., Skrabic, M., Dretar, V. ... Sikiric, P. (2022). Fourier Transform Infrared Spectroscopy Reveals Molecular Changes in Blood Vessels of Rats Treated with Pentadecapeptide BPC 157. Biomedicines, 10. (12). doi: 10.3390/biomedicines10123130

Gamulin, Ozren, et al. "Fourier Transform Infrared Spectroscopy Reveals Molecular Changes in Blood Vessels of Rats Treated with Pentadecapeptide BPC 157." Biomedicines, vol. 10, br. 12, 2022. https://doi.org/10.3390/biomedicines10123130

Gamulin, Ozren, Katarina Oroz, Luka Coric, Maria Krajacic, Marko Skrabic, Vilim Dretar, Sanja Strbe, et al. "Fourier Transform Infrared Spectroscopy Reveals Molecular Changes in Blood Vessels of Rats Treated with Pentadecapeptide BPC 157." Biomedicines 10, br. 12 (2022). https://doi.org/10.3390/biomedicines10123130

Gamulin, O., et al. (2022) 'Fourier Transform Infrared Spectroscopy Reveals Molecular Changes in Blood Vessels of Rats Treated with Pentadecapeptide BPC 157', Biomedicines, 10(12). doi: 10.3390/biomedicines10123130

Gamulin O, Oroz K, Coric L, Krajacic M, Skrabic M, Dretar V, i sur.. Fourier Transform Infrared Spectroscopy Reveals Molecular Changes in Blood Vessels of Rats Treated with Pentadecapeptide BPC 157. Biomedicines [Internet]. 04.12.2022. [pristupljeno 11.03.2025.];10(12). doi: 10.3390/biomedicines10123130

O. Gamulin, et al., "Fourier Transform Infrared Spectroscopy Reveals Molecular Changes in Blood Vessels of Rats Treated with Pentadecapeptide BPC 157", Biomedicines, vol. 10, br. 12, Prosinac 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:127:639712. [Citirano: 11.03.2025.]

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Naslov (engleski)Fourier Transform Infrared Spectroscopy Reveals Molecular Changes in Blood Vessels of Rats Treated with Pentadecapeptide BPC 157
AutorOzren Gamulin
AutorKatarina Oroz
AutorLuka Coric
AutorMaria Krajacic
AutorMarko Skrabic
AutorVilim Dretar
AutorSanja Strbe
AutorJasminka Talapko
AutorMartina Juzbasic
AutorIvan Krezic
AutorMarin Lozic
AutorVasilije Stambolija
AutorHelena Zizek
AutorIvana Jurca
AutorIvana Jurjevic
AutorAlenka Boban Blagaic
AutorAnita Skrtic
AutorSven Seiwerth
AutorPredrag Sikiric
Autorova ustanova(Klinička bolnica Merkur)
Autorova ustanova(Klinički bolnički centar Zagreb)
Autorova ustanovaSveučilište Josipa Jurja Strossmayera u Osijeku
Fakultet za dentalnu medicinu i zdravstvo Osijek
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Autorova ustanovaSveučilište u Zagrebu
Stomatološki fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Sažetak (engleski)
Recently, it was found that when confronted with major vessel occlusion and vascular failure, stable gastric pentadecapeptide BPC 157 therapy might rapidly functionally improve minor vessels to take over the function of disabled major vessels, reorganize blood flow, and compensate failed vessel function. We focused on the BPC 157 therapy effect obtained by giving 10 ng/kg ip to rats 5 min before sacrifice on the rat thoracic aorta, which we assessed with Fourier transform infrared spectroscopy (FTIR) 90 min thereafter. We applied a principal component analysis (PCA). The PCA model showed, with a clear distinction being mostly due to the PC1 score, differences between the spectra of BPC 157- and saline-treated rats. The comparison of the averaged spectra of these two groups with their differential spectrum and PC loadings allowed us to identify the parts of the FTIR spectra that contributed the most to the spectral separation of the two observed groups. The PC1 loadings and the differential spectrum showed that the main bands affecting the separation were the amid I band around 1650 cm−1, the amid II band around 1540 cm−1, and the vibrational band around 1744 cm−1. Fitting the spectral range between 1450 and 1800 cm−1 showed changes in protein conformation and confirmed the appearance of the vibrational band at 1744 cm−1. Controls had a substantially more intense vibrational band at 1744 cm−1. These spectral results showed the cells from saline-treated (control) rats to be in the early stage of cell death, while the samples from BPC 157-rats were protected. Thus, BPC 157 therapy changed the lipid contents and protein secondary structure conformation, with a rapid effect on vessels, within a short time upon application.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaBiomedicines
Brojčani podacivol. 10, br. 12, 3130
e-ISSN2227-9059
DOIhttps://doi.org/10.3390/biomedicines10123130
URN:NBNurn:nbn:hr:127:639712
Datum objave publikacije2022-12-04
URL dokumentahttps://www.mdpi.com/2227-9059/10/12/3130
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2025-03-11 09:25:37