Određivanje koncentracija IL-6 i TNF-alfa u serumu te kortizola u slini bolesnika s alergijskim kontaktnim dermatitisom šaka

disertacija

• Naslov (engleski): Determination of concentration of serum IL-6 TNF-alpha and salivary cortisol in patients with allergic contact hand dermatitis
• Autor: Nives Pondeljak
• Mentor: Liborija Lugović Mihić (mentor)
• Mentor: Dalibor Karlović (komentor)
• Član povjerenstva: Mirna Šitum (predsjednik povjerenstva)
• Član povjerenstva: Marija Buljan (član povjerenstva)
• Član povjerenstva: Romana Čeović (član povjerenstva)
• Ustanova koja je dodijelila akademski / stručni stupanj: Sveučilište u Zagrebu; Stomatološki fakultet; (Katedra za dermatovenerologiju); Zagreb
• Datum i država obrane: 2022-11-09, Hrvatska
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Kliničke medicinske znanosti; Dermatovenerologija
• Univerzalna decimalna klasifikacija (UDC): 61 - Medicina

Sažetak

Uvod: Alergijski kontaktni dermatitis (AKD) negativno utječe na kvalitetu života (KŽ). U
razjašnjavanju etiopatogenetskih čimbenika AKD­a, s psihoneuroimunološkog (PNI) aspekta,
smatra se da kronični psihički stres (PS) utječe na hipotalamo­hipozifno­adrenalnu (HHA) os i
proupalne citokine.
Metodologija: U 59 bolesnika s AKD­om šaka i 19 zdravih ispitivani je PS („Perciedved Stress
Scale“ upitnikom (PSS)), neuroendokrini (jutarnji kortizol u slini) i imunosni čimbenici
(serumski IL­6 i TNF­α), težina (“Hand Eczema Extent Score” (HEES)) i trajanje bolesti, KŽ
(„Dermatology Life Quality Index“ (DLQI)) te njihove razlike između bolesnika s atopijom i
onih bez.
Rezultati: Bolesnici s AKD­om šaka imali su niži jutarnji kortizol u slini i viši PSS (p>= 0,001)
od zdravih, bez razlike u IL­6 i TNF­α. Viši DLQI bio je povezan s višim HEES­om i PSS­om
(p=0,002 i p<0,001) te su to bili jedini prediktori KŽ. Duljina trajanja bolesti nije bila povezana
s ispitivanim čimbenicima. AKD bolesnici s atopijom imali su neznačajno viši IL­6, PSS i
DLQI te niži kortizol u slini i HEES. Kortizol u slini i serumu pozitivno su korelirali (p=0,004).
Zaključak: Ovo je prvo istraživanje PNI čimbenika u KAKD­u koje podupire postojanje
promjena u HHA osi i dokazuje utjecaj bolesti na KŽ te naglašava važnost multidisciplinarnog
pristupa bolesnicima.

Sažetak (engleski)

Introduction: Allergic contact dermatitis (ACD) is a form of late hypersensitivity reaction of
skin contact with allergens. As an inflammatory skin disease, ACD has a negative impact on
the quality of life and there is a need to elucidate the etiopathogenetic factors of the disease,
whereby using the psychoneuroimmunological (PNI) approach can be helpful. Psychological
stress (PS), as a component of PNI, leads to aggravation of the contact hypersensitivity reaction.
In response to the perception of stress, cortisol secretion is enhanced by activation of the
hypothalamic­pituitary­adrenal (HPA) axis. Furthermore, the proinflammatory cytokines
interleukin 6 (IL­6) and tumor necrosis factor alpha (TNF­α) play a role in activating the HPA
axis as well as initiating and maintaining inflammatory responses. Recent studies show that IL6, IL­1, and TNF­α values are increased in serum of patients with contact dermatitis, as well as
in keratinocyte cell culture.
Methods: The study examined the association of PNI factors (morning salivary cortisol as a
biomarker of stress, serum IL­6 and TNF­α, stress intensity with "Perceived Stress Scale" (PSS)
questionnaire, quality of life of dermatology patients with "Dermatology Life Quality Index"
(DLQI)) with the disease severity evaluated using the Hand Eczema Extent Score (HEES) and
the duration of disease in hand ACD patients, as well as the differences between patients with
ACD and atopic disease and those without atopy.
Results: This study examined the association of PNI factors (morning salivary cortisol as a
biomarker of stress, serum IL­6 and TNF­α, stress intensity with "Perceived Stress Scale" (PSS)
questionnaire, quality of life of dermatology patients with "Dermatology Life Quality Index"
(DLQI)) with the disease severity evaluated using the Hand Eczema Extent Score (HEES) and
the duration of disease in hand ACD patients, as well as the differences between patients with
ACD and atopic disease and those without atopy.
Conclusion: This study is the first so far, to our knowledge, in which a detailed analysis of PNI
factors in patients with hand ACD was conducted. The results show that patients with ACD
have lower values of morning cortisol in saliva and higher stress intensity, which indicates
changes in the balance of the HPA axis and indirectly affects the quality of life and severity of
the clinical picture of teh disease. The results of the research provide more knowledge about
ACD and contribute to and emphasize the importance of a multidisciplinary approach to
treatment, thus improving the quality of life of these patients.

• Ključne riječi: alergijski kontaktni dermatitis; proteini i peptidi u slini; psihički stres; receptor interleukin-6; nekrotizirajući tumor faktor-alfa
• Ključne riječi (engleski): Allergic Contact Dermatitis; Salivary Proteins and Peptides; Psyhological Stress; Interleukin-6 Receptors; Tumor Necrosis Factor-alpha
• Jezik: hrvatski
• URN:NBN: urn:nbn:hr:127:517094
• Datum promocije: 2022
• Studijski program: Naziv: Dentalna medicina; Vrsta studija: sveučilišni; Stupanj studija: poslijediplomski doktorski; Akademski / stručni naziv: doktor/doktorica znanosti, područje biomedicine i zdravstvo, polje dentalna medicina (dr.sc.)
• Vrsta resursa: Tekst
• Način izrade datoteke: Izvorno digitalna
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2022-11-21 12:27:16