Primjena hladne atmosferske plazme u terapiji osteonekroze čeljusti

master's thesis

• Title (english): Use of cold atmospheric plasma in therapy of osteonecrosis of the jaw
• Author: Marta Hrelja
• Mentor: Božidar Pavelić (mentor)
• Committee member: Božidar Pavelić (predsjednik povjerenstva)
• Committee member: Nada Galić (član povjerenstva)
• Committee member: Tihomir Kuna (član povjerenstva)
• Granter: University of Zagreb; School of Dental Medicine; Zagreb
• Defense date and country: 2023-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Dental Medicine

Abstract

Plazma je djelomično ili potpuno ioniziran plin sastavljen od mješavine brojnih aktivnih komponenti: iona, fotona, slobodnih elektrona, radikala te raznih drugih pobuđenih i neutralnih čestica. Hladna atmosferska plazma (engl. cold atmospheric plasma, CAP) je vrsta plazme čija je klinička primjena moguća jer se stvara pri atmosferskome tlaku i temperaturi ispod 40 ⁰C. Stvara se dovođenjem određene vrste energije plinu/plinovima poput argona i helija te ambijentalnom zraku. Postoji više različitih uređaja koji stvaraju plazmu, a za medicinsku se primjenu najčešće upotrebljavaju uređaji s dielektričnim barijernim izbojem (DBD) i plazma - mlazni uređaji (engl. plasma jets). Plazma je našla široku primjenu u području biomedicinskih znanosti uključujući stomatologiju. U ovom radu naglasak je stavljen na moguću primjenu hladne atmosferske plazme u terapiji osteonekrotičnih žarišta. Osteonekroza čeljusti patološko je stanje oštećenja i progresivnog odumiranja koštanog tkiva čeljusti. Može se podijeliti na osteoradionekrozu, medikamentoznu osteonekrozu, traumatsku, netraumatsku i spontanu osteonekrozu. Glavni klinički pokazatelj svih vrsta osteonekroza je eksponirano koštano tkivo koje ne cijeli kroz dulje razdoblje. Prema kliničkoj slici i radiološkom nalazu razlikuju se različiti stadiji bolesti koji predstavljaju smjernice za odabir terapije. Primjena CAP-a spada u relativno novije oblike terapije osteonekroze čeljusti. Ona se upotrebljava kao dodatak osnovnoj terapiji kako bi ubrzala cijeljenje osteonekrotičnih lezija najviše zahvaljujući antiseptičkom učinku koji omogućuje postizanje sterilnih uvjeta u leziji, a smatra se i da direktno potiče zacjeljivanje i regeneraciju osteonekrozom zahvaćenih tkiva putem stimuliranja proliferacije i migracije ključnih stanica. Iako nedovoljno istražena terapijska metoda, pokazuje vrlo dobre rezultate u tretiranju osteonekrotičnih žarišta i za sada nema zabilježenih negativnih posljedica na zdrava tkiva.

Abstract (english)

Plasma is a partially or fully ionized gas composed of a mixture of numerous active components: ions, photons, free electrons, radicals, and various other charged and neutral particles. Cold atmospheric plasma (CAP) is a type of plasma suitable for clinical applications because it is generated at atmospheric pressure and temperatures below 40°C. It can be generated by applying a specific type of energy to a gas or a combination of gases such as argon, helium, and ambient air. There are several different devices that generate plasma, with dielectric barrier discharge (DBD) devices and plasma jet devices being most commonly used for medical purposes. Plasma has found wide application range in the field of biomedical sciences, including dentistry. This study emphasizes the potential of plasma in healing osteonecrotic lesions. Osteonecrosis of the jaw is a pathological condition characterized by damage and progressive necrosis of jawbone tissue. It is categorized into osteoradionecrosis, medication-related, trauma-induced, non-traumatic, and spontaneous osteonecrosis. The main clinical indicator of all types of osteonecrosis is exposed bone tissue that does not heal over an extended period of time. Based on the clinical presentation and radiological findings, different stages of disease can be identified, which then serve as guidelines for selecting the appropriate therapy. CAP represents one of the more recent therapeutic approaches for treating osteonecrosis of the jaw. It is used alongside conventional therapy to accelerate the healing of osteonecrotic lesions, primarily due to its antiseptic properties that establish a sterile environment in the affected area. It is also thought to directly stimulate the healing and regeneration of osteonecrosis-affected tissues by promoting the proliferation and migration of key wound-related cells. Although it is a relatively understudied therapeutic method, it has shown very promising results in the treatment of osteonecrotic lesions, with no recorded negative effects on healthy tissues so far.

• Keywords: hladna atmosferska plazma; osteonekroza čeljusti; terapija
• Keywords (english): cold atmospheric plasma; jaw osteonecrosis; therapy
• Language: croatian
• URN:NBN: urn:nbn:hr:127:502377
• Study programme: Title: dental medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica dentalne medicine (doktor/doktorica dentalne medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-09-20 15:48:15