Matične stanice u regeneraciji pulpo-dentinskog kompleksa

master's thesis

• Title (english): Stem cells in regeneration of dentine-pulp complex
• Author: Martina Perušina Arbanas
• Mentor: Anja Baraba (mentor)
• Committee member: Anja Baraba (predsjednik povjerenstva)
• Committee member: Nada Galić (član povjerenstva)
• Committee member: Ivana Miletić (član povjerenstva)
• Granter: University of Zagreb; School of Dental Medicine; (Department of Endodontics and Restorative Dentistry); Zagreb
• Defense date and country: 2021-09-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Dental Medicine

Abstract

U slučaju ireverzibilnog pulpitisa ili nekroze pulpe, klasična terapija korijenskog kanala podrazumijeva endodontsko liječenje zuba. Takva terapija ne omogućava cijeljenje i regeneraciju pulpnog tkiva jer se ono tijekom endodontskog zahvata uklanja kemomehanički i tako obrađeni prostor hermetički se brtvi materijalima za punjenje korijenskih kanala. Regenerativna endodoncija alternativna je opcija koja regenerira pulpno-dentinski kompleks, a uključuje tri osnovne komponente: matične stanice koje se mogu diferencirati u stanice pulpe, bioaktivne molekule – čimbenike rasta za pokretanje i promicanje stanične diferencijacije te nosač koji mehanički podupire stanice i pruža im povoljan okoliš. Izvori matičnih stanica iz zubnih tkiva mogu biti zubna pulpa, parodontni ligament, zubni folikul, gingiva ili alveolarna kost, a najčešće se koriste matične stanice zubne pulpe zbog njihove jednostavnosti dobivanja i izuzetne sposobnosti diferencijacije. Prema dostupnim istraživanjima, korištenjem matičnih stanica stvaraju se tkiva nalik pulpi i dentinu, pulpa je vaskularizirana i inervirana, stvorene stanice slične su odontoblastima koji stvaraju mineralizirano tkivo. Međutim, regenerirana tkiva nemaju nužno originalnu pulpnu arhitekturu i funkciju. Osim toga potencijalni su problemi: potrebna veličina apikalnog otvora, svojstava čimbenika rasta i nosača, metoda izolacije i uzgoja matičnih stanica, kliničko rukovanje, izvedivost i troškovi liječenja. Buduća in vivo istraživanja trebala bi pokazati uspješnost i potencijalne komplikacije transplantacije matičnih stanica.

Abstract (english)

In the case of irreversible pulpitis or pulp necrosis, classical therapy involves root canal treatment. Such therapy does not enable healing and regeneration of pulp tissue because pulp or pulp remnants are removed chemomechanically during the endodontic treatment where endodontic space is hermetically sealed using root canal filling materials. Regenerative endodontics is an alternative option used for regeneration of dentine-pulp complex and it is based on three basic components: stem cells that can differentiate into pulp cells, bioactive molecules - growth factors, that initiate and promote cell differentiation and a scaffold that mechanically supports cells and provides them with favorable environment. Sources of stem cells from dental tissues are dental pulp, periodontal ligament, dental follicle, gingiva or alveolar bone. Dental pulp stem cells are most commonly used because they are easily accessible and have an exceptional ability to differentiate. According to available research, by using stem cells, pulp- and dentin-like tissues are obtained, the pulp is vascularized and innervated and the differentiated cells are similar to odontoblasts and produce mineralized tissue. However, regenerated tissues do not necessarily have the original pulp architecture and function. In addition, potential problems are: apical foramen size, growth factor and scaffold properties, stem cells isolation and culture methods, clinical handling, feasibility and treatment costs. Future in vivo studies should demonstrate the success and potential complications of stem cell transplantation.

• Keywords: zubna pulpa; matične stanice; međučelijski signalni peptidi i proteini
• Keywords (english): Dental Pulp; Stem Cells; Intercellular Signaling peptides and Proteins
• Language: croatian
• URN:NBN: urn:nbn:hr:127:211415
• Study programme: Title: dental medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica dentalne medicine (doktor/doktorica dentalne medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-09-17 08:56:10