Određivanje fluorida u sredstvima za prevenciju karijesa

master's thesis

• Title (english): ANALYSIS OF FLUORIDE CONCENTRATION IN CARIES-PREVENTIVE AGENTS
• Author: Marko Turkalj
• Mentor: Kristina Peroš (mentor)
• Committee member: Kristina Peroš (predsjednik povjerenstva)
• Committee member: Ivana Šutej (član povjerenstva)
• Committee member: Lea Vuletić (član povjerenstva)
• Granter: University of Zagreb; School of Dental Medicine; (Chair of Pharmacology); Zagreb
• Defense date and country: 2019-07-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Dental Medicine

Abstract

Zbog prevencije nastanka i liječenja inicijalnih karijesnih lezija godinama se upotrebljavaju zubne paste. U njima najčešće nalazimo različite fluoridne spojeve kao remineralizacijski aktivne tvari. Njihova učinkovitost ovisi o otpuštanju fluoridnog (F-) iona. Također, postoje i remineralizacijski sustavi bez fluora, temeljeni na ionima kalcija i fosfata koji se dodaju zubnim pastama. Svrha ovog istraživanja bila je izmjeriti koncentracije fluoridnih iona u vodenim otopinama zubnih pasti te ih usporediti s deklariranim koncentracijama. Odabrano je šest zubnih pasti s različitim aktivnim sastojcima (natrijev fluorid, aminofluorid, natrijev monofluorofosfat, aminofluorid i kositrov fluorid, kalcijev fluorid, natrijev fluorid i kazein fosfopeptid-amorfni kalcijev fosfat (CPP-ACP)). Od svake zubne paste pripremljena su po dva uzorka za mjerenje tako da se jedan gram zubne paste izmjerio analitičkom vagom, a zatim otopio u redestiliranoj vodi. Potom su se koncentracije fluoridnih iona izmjerile ion-selektivnom elektrodom, metodom propisanom normom ISO 19448:2018. Izmjerene koncentracije fluoridnih iona u pastama s aminofluoridima i natrijevim fluoridom odgovarale su deklariranoj koncentraciji. Kalcijev fluorid i natrijev monofluorofosfat nisu disocirali u vodenoj otopini, stoga su koncentracije u zubnim pastama koje ih sadrže bile niže od deklariranih. Takvi rezultati odgovaraju podatcima iz dostupne literature. Izmjerene koncentracije F- iona u zubnoj pasti s NaF-om i CPP-ACP-om te pasti s kositrovim fluoridom i aminofluoridom nisu odgovarale deklariranima. Fluoridni ioni iz zubne paste koja sadrži NaF i CPP-ACP kompleks, unatoč tome što se u proizvodu nalaze u obliku NaF-a, nisu u vodenoj otopini slobodni, već dolazi do interakcije između CPP-ACP-a i F- iona.

Abstract (english)

Fluoride compounds are used in dentistry primarily for caries prevention. Commercially available toothpastes contain a wide variety of remineralization compounds. Their effectiveness depends on the delivery of free or soluble fluoride ions (F-). Apart from them, there are also non-fluoride remineralization systems exist. They are based on calcium and phosphate ions, which are added to toothpastes. The aim of this study was to determine the concentrations of fluoride ions in aqueous solutions of toothpastes and to compare them to the label amount of fluoride concentration. Six toothpastes were chosen for the study. They contained different fluoride compounds: sodium fluoride (NaF), amine fluoride, sodium monofluorophosphate, amine fluoride/stannous fluoride, calcium fluoride, sodium fluoride/casein phosphopeptide-amorphous calcium phosphate complex (CPP-ACP). Two samples were prepared from each of the toothpastes. A single gram of the toothpaste was measured using the analytical scale and then dissolved in redistilled water. Next, the amount of F- ion was determined by use of ion-selective electrode (ISO 19448:2018 standard). The measured concentrations of F- ions in toothpastes containing amine fluorides and sodium fluoride matched the label concentration. Calcium fluoride and sodium monofluorophosphate didn’t dissociate in redistilled water, therefore the measured concentrations were lower than the label amount of fluorides in toothpastes containing them. Those results correspond to the available literature data. The measured concentrations of F- ions in the toothpastes with NaF/CPP-ACP and stannous fluoride/amine fluoride didn’t match the label concentration. Fluoride ions from the toothpaste containing NaF and CPP-ACP complex, despite the product containing NaF, didn’t dissociate in an aqueous solution. That indicates ion interactions between sodium fluoride and CPP-ACP complex.

• Keywords: elektroda; fluor; zubne paste; remineralizacija
• Keywords (english): electrode; fluorine; toothpaste; remineralization
• Language: croatian
• URN:NBN: urn:nbn:hr:127:981886
• Study programme: Title: dental medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica dentalne medicine (doktor/doktorica dentalne medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-01-30 09:25:42