Primjena matičnih stanica mliječnih zuba u regeneraciji tkiva

master's thesis

• Title (english): Application of stem cells from deciduous teeth in tissue regeneration
• Author: Sara Čekalović
• Mentor: Dubravka Negovetić-Vranić (mentor)
• Committee member: Dubravka Negovetić-Vranić (predsjednik povjerenstva)
• Committee member: Andreja Carek (član povjerenstva)
• Committee member: Eva Klarić Sever (član povjerenstva)
• Granter: University of Zagreb; School of Dental Medicine; (Department of Paediatric and Preventive Dentistry); Zagreb
• Defense date and country: 2018-06-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Dental Medicine; Children's and Preventive Dental Medicine

Abstract

Matične su stanice nediferencirane vrste stanica koje imaju sposobnost diferencijacije u specijalizirane stanice. Njihove glavne osobine su samoobnovljivost i potentnost. Razlikujemo embrionalne matične stanice, koje se izoliraju iz embrija u stadiju blastociste, i odrasle matične stanice koje su prisutne u različitim vrstama tkiva. Ranih godina 21. stoljeća otkrivaju se dentalne matične stanice od kojih razlikujemo matične stanice zubne pulpe, parodontnog ligamenta, apikalne papile, dentalnog folikula i mliječnih zuba. Matične stanice mliječnih zuba pokazuju veći broj dioba i mogućnost proliferacije u odnosu na ostale vrste dentalnih matičnih stanica. In vitro i in vivo istraživanja pokazuju njihovu mogućnost diferencijacije u adipogene, hondrogene, angiogene, osteogene i mnoge druge specijalizirane vrste stanica. Njihova sposobnost u regeneraciji i obnavljanju tkiva omogućuje njihovu terapeutsku primjenu kod bolesti stomatognatog sustava, ali i teških sistemnih bolesti. U novije vrijeme došlo je do razvoja banaka matičnih stanica koje nude pacijentima pohranu matičnih stanica mliječnih zuba i njihovo korištenje u slučaju da to donoru u budućnosti zatreba. Prikupljanje mliječnih zuba potrebno je napraviti u stomatološkoj ordinaciji, pod vodstvom doktora koji će procijeniti koji zub je kandidat za uspješnu izolaciju matičnih stanica. Pohranjeni zub šalje se u banku nakon čega slijedi laboratorijska obrada. Vjerujemo da će ovakve usluge u skoroj budućnosti biti dostupne u svakoj ordinaciji, a otvaranje banke u Hrvatskoj zasigurno bi omogućilo korištenje ove usluge većem broju pacijenata.

Abstract (english)

Stem cells are an undifferentiated type of cells that have the ability to differentitate to specialized cells. Their main features are self-renewal and potency. We differentiate embryonic stem cells which isolate themsleves from embryos in the blastocyst and adult stem cells present in different tissue types. In the early 21st century, dental stem cells were discovered, which can be distinguished as stem cells of dental pulp, periodontal ligaments, apical papillae, dental follicles and deciduous teeth. Stem cells of deciduous teeth show a greater number of diploids and proliferation than the other types of stem cells. In vitro and in vivo studies show their ability to differentiate to adipogenic, hondrogenic, angiogenic, osteogenic and many other specialized types of cells. Their ability to regenerate and repair tissue allows their therapeutic application in the illnesses of the stomatognathic system as well as severe systemic diseases. More recently, the development of stem cells banks have been founded to provide patients with the storage of stem cells for their teeth and their use in the event that donor needs them in the future. The collection of deciduous teeth needs to be done at a dental office under the guidance of a doctor who will assess which tooth is a candidate for the successful stem cell isolation. The stored tooth is then sent to the bank, followed by laboratory processing. We believe that these kinds of services will be available in every dental office in the near future, and opening up a dental bank in Croatia would surely enable these services to a large number of patients.

• Keywords: matične stanice; matične stanice mliječnih zuba; prikupljanje; pohrana; terapeutska primjena
• Keywords (english): stem cells; stem cells of deciduous teeth; collection; storage; therapeutic use
• Language: croatian
• URN:NBN: urn:nbn:hr:127:566524
• Study programme: Title: dental medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica dentalne medicine (doktor/doktorica dentalne medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-11-22 09:36:37