In vivo assessment of genotoxicity in buccal cells of children undergoing tooth restoration

Scientific paper - Original scientific paper

• Author: Lidia Gavić
• Author: Kristina Goršeta
• Author: Domagoj Glavina
• Author: Davor Željezić
• Author: Nada Galić; MBZ: 192110
• Author: Antonija Tadin
• Author's institution: Institute for Medical Research and Occupational Health
• Author's institution: (Zagreb University Hispital Center)
• Author's institution: University of Split; School of Medicine
• Author's institution: University of Zagreb; School of Dental Medicine
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Dental Medicine

Abstract (english)

Objectives: Whereas dental materials came in direct or close contact with oral tissue, it is a great concern about the biocompatibility of those materials. This study was performed to evaluate possible DNA damage to buccal cells exposed to dental materials.
Methods: This prospective, longitudinal clinical study was conducted over a three months period. Class II restorations were placed in 60 young patients with no previous filling using one of three tested dental materials (two glass ionomers; Ketac Molar and Ionofil Molar and one compomer material; Twinky Star). DNA damage was analysed by micronucleus assays, in buccal exfoliated epithelial cells.
Results: In patients treated with Ketac Molar, a significant frequency of micronuclei (p = 0.027) and binucleated cells in samples taken 30 days following restoration (p = 0.029) was confirmed. In patients treated with Twinky Star, a statistically significant increase in the number of binucleated cells in samples taken after 7 and 30 days following restoration (p = 0.001 and p < 0.001, respectively) was observed. In all samples collected 90 days after treatment, there was no statistical difference in the number of any cell changes.
Conclusion: In this study long-term biocompatibility of tested materials was confirmed. Glass ionomers and compomers are widely used materials in paediatric dentistry, and this study has proved their safety for usage in children.

• Keywords (english): Compomers; Dental materials; DNA damage; Genotoxicity test; Glass ionomer cements; Restorative dentistry
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Central European Journal of Public Health
• Numbering: vol. 27, no. 4, pp. 312-319
• p-ISSN: 1210-7778
• e-ISSN: 1803-1048
• DOI: https://doi.org/10.21101/cejph.a5216
• URN:NBN: urn:nbn:hr:127:502600
• Publication: 2019-12-31
• Document URL: http://cejph.szu.cz/doi/10.21101/cejph.a5216.html
• Type of resource: Text
• Access conditions: Open access
• Created on: 2024-10-24 07:34:41