Influence of saliva on the results of global laboratory coagulation tests

Scientific paper - Original scientific paper

• Author: B Špiljak
• Author: L Šimunović
• Author: I Lapić
• Author: D Rogić
• Author: S Špalj
• Author: L Vuletić
• Author's institution: University of Rijeka
• Author's institution: (Clinical Hospital Center Rijeka)
• Author's institution: (Zagreb University Hispital Center)
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry
• Author's institution: University of Zagreb; School of Dental Medicine
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Dental Medicine

Abstract (english)

Background: Oral wounds inevitably come into contact with saliva which can affect the time needed for bleeding to stop. The influence of saliva can be non-specific, related to dilution of blood, and/or mediated by salivary factors that affect haemostasis directly. The aim of this study was to assess if mixing blood with an individual's saliva would affect the rate of its coagulation measured by global coagulation tests, prothrombin time (PT) and activated partial thromboplastin time (APTT). Methods: The study included 30 healthy non-smoking volunteers. Paired blood and unstimulated saliva samples were obtained from each participant and PT and APTT were determined in blood, blood + saliva and blood + water mixtures. Coagulation tests were performed using the mechanical clot detection method. Results: PT was significantly longer in both blood + saliva and blood + water mixtures compared to blood alone. APTT was significantly longer only in blood + water mixture compared to blood. Conclusions: Similarly prolonged PT in both mixtures suggests that both saliva and water prolong coagulation evenly due to their non-specific effect of blood dilution. The finding that APTT was significantly prolonged only when blood was mixed with water could indicate presence of tissue factor in saliva, however, in a concentration too low to influence the results of PT.

• Keywords (english): Activated partial thromboplastin time; blood coagulation; blood coagulation tests; prothrombin time; unstimulated saliva
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Australian Dental Journal
• Numbering: vol. 65, no. 3, pp. 205-209
• p-ISSN: 0045-0421
• e-ISSN: 1834-7819
• DOI: https://doi.org/10.1111/adj.12753
• URN:NBN: urn:nbn:hr:127:172185
• Publication: 2020-03-27
• Document URL: https://onlinelibrary.wiley.com/doi/10.1111/adj.12753
• Type of resource: Text
• Access conditions: Open access
• Created on: 2024-10-22 10:05:58