Utjecaj lasera na kvalitetu brtvljenja bioaktivnih materijala za retrogradno punjenje korijenskih kanala

master's thesis

• Title (english): Effect of laser on the sealing ability of bioactive retrofilling materials
• Author: Ružica Lucić
• Mentor: Ivona Bago Jurič (mentor)
• Committee member: Ivona Bago Jurič (predsjednik povjerenstva)
• Committee member: Ivica Anić (član povjerenstva)
• Committee member: Božidar Pavelić (član povjerenstva)
• Granter: University of Zagreb; School of Dental Medicine; (Department of Endodontics and Restorative Dentistry); Zagreb
• Defense date and country: 2016-09-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Dental Medicine; Endodonics and Restorative Dentistry

Abstract

Svrha ovog in vitro istraživanja je bila usporediti kvalitetu apikalnog brtvljenja
mineral trioksid agregata (MTA) i biokeramičkog punila (Bioceramic, Root Repair
Material, BC-RRM) u kavitetima izrađenim Er, Cr:YSGG laserom i UZV-om.
U istraživanju je korišteno 48 humanih, ekstrahiranih jednokorijenskih zuba.
Nakon ProTaper Universal tehnike instrumentacije, apikalni dijelovi korijenova su
odrezani za 3 mm. Uzorci su nasumično podijeljeni dvije skupine ovisno o tehnici
izrade retrogradnog kaviteta: Skupina 1: Er, Cr:YSGG laser, Skupina 2: ultrazvučna
tehnika. U svakoj skupini, retrogradni kaviteti su u polovine uzoraka napunjeni s
MTA-om, a u druge polovine s BC-RRM-om. U pozitivnoj kontrolnoj skupini
retrogradni kaviteti su napunjeni s privremenim materijalom, a u negativnoj
ružičastim voskom te je čitava površina premazana lakom. Uzorci su fiksirani u
plastične Eppendorf epruvete i sterilizirani u plazmi. Korijenski kanali su
kontaminirani suspenzijom E. faecalis-a, a epruvete napunjene s fetalnim bovinim
serumom. Nakon 30 dana inkubacije, iz korijenskih kanala su prikupljeni
mikrobiološki uzorci za metodu culture i test reakcije lančane polimeraze (PCR).
Nije bilo značajne razlike između BC-RRM i MTA materijala u kavitetima
izrađenim ultrazvučnim nastavkom (p > 0,05). Slične rezultate pokazao je MTA u
kavitetima izrađenim Er, Cr:YSGG laserom (p > 0,05). Značajno veći broj bakterija
otkriven je u skupini BC-RRM materijala punjenog u kavitetima izrađenim Er,
Cr:YSGG laserom (p < 0.001).
Nema razlike u kvaliteti apikalnog brtvljenja MTA materijala u retrogradnim
kavitetima izrađenim Er, Cr:YSGG laserom i UZV-om. Najveće apikalno
propuštanje zabilježeno je u kavitetima izrađenim laserom i punjenim BC-RRM-om.

Abstract (english)

The aim of the in vitro study was to compare the apical sealing ability of Total
Fill Bioceramic Root Repair Material (BC-RRM) and Mineral Trioxide Aggregate
(MTA) regarding the retrogade preparation technique used: ultrasonic and Er,
Cr:YSGG laser. The study sample consisted of 48 human single-rooted teeth, which
were instrumented with ProTaper Universal rotary technique. After root-end
resection (3 mm), the root specimens were divided in two groups according the
retrogade preparation technique used: Group 1: ultrasonic technique, Group 2: Er, Cr
:YSGGlaser. In each group, the half of the retrogade cavities was filled with BC
RRM and another half with MTA The positive controls were filled with temporary
material. In the negative controls, with utility wax. The outer root surfaces were
covered with layers of nail varnish. The specimens were mounted in tubes and
sterilized in plasma. The root canals were inoculated with E. faecalis suspension, and
the tubes were filled with fetal bovine serum. After 30 days, the canals were sampled,
cultured and the colony forming units (CFUs) were counted. The presence of E.
faecalis was also determined by PCR.
There was no significant difference between BC-RRM i MTA in the ultrasonic
groups (p > 0,05). Similar results showed MTA in cavities made with Er, Cr:YSGG
laser (p> 0.05). A significantly higher number of bacteria was detected in the laser
group with BC-RRM filling (p <0.001).
There is no difference in the apical seling ability of MTA in retrograde cavities
made with Er, Cr: YSGG laser and ultrasound. BC-RRM showed more leakage when
used in retro-cavities prepared by Er, Cr: YSGG laser.

• Keywords: korijenski kanal; sredstva za punjenje; laseri
• Keywords (english): root canal filling materials; lasers
• Language: croatian
• URN:NBN: urn:nbn:hr:127:597254
• Study programme: Title: dental medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica dentalne medicine (doktor/doktorica dentalne medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-11-15 11:06:09