Utjecaj različitih irigacijskih protokola na snagu sveze samoadherirajućeg kompozitnog cementa i radikularnog dentina

master's thesis

• Title (english): The impact of different irrigation protocols on the bond strength of self-adhering composite cement to radicular dentin
• Author: Mihaela Vrebac
• Mentor: Ana Ivanišević (mentor)
• Committee member: Ana Ivanišević (predsjednik povjerenstva)
• Committee member: Eva Klarić Sever (član povjerenstva)
• Committee member: Jurica Matijević (član povjerenstva)
• Granter: University of Zagreb; School of Dental Medicine; (Department of Endodontics and Restorative Dentistry); Zagreb
• Defense date and country: 2023-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Dental Medicine; Endodonics and Restorative Dentistry

Abstract

Cilj je istraživanja bio ispitati smičnu snagu sveze samoadherirajućeg kompozitnog cementa na radikularni dentin s obzirom na različite irigacijske protokole koji su prethodili adheziji – natrijevim hipokloritom (HIPO), etilen-diamin-tetra octenom kiselinom (EDTA), etidronskom kiselinom s HIPO (HEDP), fiziološkom otopinom (FO) i klorheksidinom (CHX). Od ekstrahiranih intaktnih impaktiranih trećih kutnjaka ispiljeni su izbrusci korijenskog dentina (N=58), uloženi u akrilat, ispolirani te nasumično podijeljeni u 5 eksperimentalnih skupina (N=8–12) i kontrolnu skupinu (N=8). U eksperimentalnim skupinama uzorci dentina tretirani su tijekom dviju minuta različitim irigacijskim protokolima: 1) HIPO/EDTA/HIPO, 2) HEDP, 3) HIPO/EDTA/CHX, 4)HIPO/EDTA/FO i 5)HEDP/FO. Kontrolni uzorci pohranjeni su u destiliranoj vodi i nisu tretirani dezinficirajućim otopinama. Nakon toga na osušen dentin apliciran je dvostruko stvrdnjavajući samoadherirajući kompozitni cement (GC G-CEM LinkForce, GC Tokyo, Japan) pomoću pripadajućih kalupa Ultra Tester kidalici (Ultradent Products, SAS Institute Inc., Cary, NC, USA). Smična snaga sveze samoadherirajućeg cementa za radikularni dentin određena je pomoću kidalice Ultra Tester pri konstantnoj brzini 1 mm/min do trenutka odvajanja cementa od dentina. Rezultati su analizirani ANOVA statističkim testom te Post-hoc Games-Howell testom. Razina statističke značajnosti bila je α=0,05. Sve eksperimentalne skupine osim HEDP i HEDP/FO pokazale su značajno manju snagu sveze u odnosu na kontrolnu skupinu (p<0.05). U granicama ovog in vitro istraživanja može se zaključiti da primijenjene irigacijske otopine smanjuju snagu sveze kompozitnog cementa za radikularni dentin, a irigacija etidronskom kiselinom prije adhezivnog cementiranja predstavlja najpovoljniji protokol s obzirom na smanjenje smične snage sveze.

Abstract (english)

The aim of the study was to examine the shear bond strength of self-adhering composite cement to radicular dentin under different irrigation protocols. The study compared the effects of various irrigation solutions, including sodium hypochlorite, ethylene-diamine-tetra acetic acid, etidronic acid with hypochlorite, saline, and chlorhexidine. In this study, 58 radicular dentin slabs were sectioned from extracted intact third molars, embedded in acrylate, polished, and randomly divided into three experimental groups (N=8- 12) and one control group (N=8). Each experimental group underwent a different irrigation protocol for two minutes: 1) HIPO/EDTA/HIPO, 2) HEDP, 3) HIPO/EDTA/CHX, 4) HIPO/EDTA/FO, and 5) HEDP/FO. The control samples were not treated with any disinfectant solution and were stored in distilled water. After drying, dual-curing self-adhering composite cement was applied to the radicular dentin using the corresponding Ultra Tester cutter molds. The shear bond strength of the self-adhering cement to radicular dentin was measured using an Ultra Tester until the cement separated from the dentin. The results for the shear bond strength in MPa were analyzed using the ANOVA statistical test and Post-hoc Games-Howell test, with a significance level of α=0,05. Except for HEDP and HEDP/FO, all experimental groups showed significantly lower bond strength compared to the control group (p < 0,05). Within the limitations of this in vitro study, it can be concluded that the applied irrigation solutions reduce the bond strength of composite cement to radicular dentin. Irrigation with etidronic acid before adhesive cementation is the most favorable protocol, as the decrease in bond strength is not statistically significant.

• Keywords: korijenski kanal; etidronska kiselina; smična snaga; samoadherirajući kompozitni cement; dentin
• Keywords (english): root canal; etidronic acid; shear strength; self-adhering composite cement; dentin
• Language: croatian
• URN:NBN: urn:nbn:hr:127:365239
• Study programme: Title: Integrated undergraduate and graduate university study of Dental Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica dentalne medicine (doktor/doktorica dentalne medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2024-07-14
• Created on: 2023-07-07 08:56:55