Otpornost bakterijskog biofilma na djelovanje antimikrobnih sredstava

master's thesis

• Title: Otpornost bakterijskog biofilma na djelovanje antimikrobnih sredstava : analiza konfokalnom laserskom mikroskopijom
• Title (english): Antimicrobial efficacy of lasers in the disinfection and bacterial biofilm removal
• Author: Merima Balić
• Mentor: Ivica Anić (mentor)
• Committee member: Ivica Anić (predsjednik povjerenstva)
• Committee member: Ivona Bago Jurič (član povjerenstva)
• Committee member: Božidar Pavelić (član povjerenstva)
• Granter: University of Zagreb; School of Dental Medicine; (Department of Endodontics and Restorative Dentistry); Zagreb
• Defense date and country: 2016-06-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Dental Medicine; Endodonics and Restorative Dentistry

Abstract

Tradicionalnim kemijsko-mehaničkim postupcima čišćenja korijenskih kanala ne može se postići uspješna dezinfekcija cijeloga endodontskog prostora. Od 40 do 60 % korijenskih kanala ostaje i dalje inficirano nakon endodontske mehaničke obrade i ispiranja natrijevim hipokloritom. Zaostale bakterije u nepristupačnim dijelovima endodontskog prostora i u dentinskim tubulusima mogu spriječiti cijeljenje periapikalnoga procesa ili povećati rizik od nastanka perzistirajuće upale. Zbog specifične interakcije lasera s biološkim tkivima, posljednja dva desteljeća istražuje se njihova primjena u dezinfekciji korijenskih kanala zuba. Predstavljena je učinkovitost diodnog, Nd:YAG i erbij lasera u dezinfekciji dentina korijenskog kanala. Objašnjeni su načini primjene lasera u kombinaciji s otopinama za ispiranje te metode evaluacije njihove učinkovitosti. Na temelju dosadašnjih istraživanja dokazano je da laseri ostvaruju baktericidni učinak u korijenskom kanalu. S obzirom na to da ne mogu potpuno zamijeniti natrijev hipoklorit, preporučuju se kao dodatna tehnika dezinfekcije na kraju kemijsko-mehaničke obrade korijenskog kanala.

Abstract (english)

The traditional chemical-mechanical procedures for root canal treatment are not completely successful in the disinfection of the endodontic space. After mechanical instrumentation and the use of sodium hypochlorite as an irrigant, 40-60% of dentin surfaces still contain microorganisms. Persisting microorganisms in dentinal tubules and root canal irregularities may cause reinfection and prevent healing of periapical lesions. In the last two decades, many papers on laser use in endodontics have been published due to the specific interaction of lasers with biological tissues. Erbium and Nd:YAG wavelengths can be used for root canal debridement and cleaning, including for laser-activated irrigation and photon-initiated photoacoustic streaming. This paper reviews the literature on efficacy of the diode laser, Nd:YAG and the erbium laser in root canal disinfection.
Based on recent literature, it can be concluded that laser irradioation has the potential to kill microorganisms, but there is no sufficent evidence to suggest lasers are superior to the traditional endodontic treatment. Hence, the use of lasers is recommended not as alternative to NaOCl, but as an adjunct to traditional disinfection protocols.

• Keywords: diodni laser Nd:YAG; erbij laser; dezinfekcija korijenskih kanala zuba
• Keywords (english): diode laser Nd:YAG; erbium laser; root canal disinfetion
• Language: croatian
• URN:NBN: urn:nbn:hr:127:382656
• Study programme: Title: dental medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica dentalne medicine (doktor/doktorica dentalne medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-09-07 10:36:48