Utjecaj kalcija i fosfata na odlaganje fluorida u caklinu

master's thesis

• Title (english): The effect of calcium and phosphate on fluoride uptake on enamel
• Author: Ana Marija Jurinić
• Mentor: Kristina Peroš (mentor)
• Committee member: Kristina Peroš (predsjednik povjerenstva)
• Committee member: Ivana Šutej (član povjerenstva)
• Committee member: Eva Klarić Sever (član povjerenstva)
• Granter: University of Zagreb; School of Dental Medicine; (Chair of Pharmacology); Zagreb
• Defense date and country: 2018-07-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Dental Medicine

Abstract

Svrha ovog in vitro istraživanja je procijeniti učinak sline pušača u kombinaciji s preparatom koji sadrži kalcij i fosfate na odlaganje flourida na zubnu caklinu, uz pretpostavku da postoji razlika u koncentraciji kalcija u slini pušača i nepušača.
Sa svakog od deset ekstrahiranih humanih zuba odrezana su četiri caklinska bloka i podijeljeni u četiri skupine (A, B, C, D). Uzorke nestimulirane sline doniralo je 12 zdravih muškaraca, šest pušača i šest nepušača. Zubna krema korištena u istraživanje je MI Paste Plus (GC, Alsip, Illinois, SAD). Caklinski blokovi skupine A tretirani su slinom pušača, a zatim smjesom zubne kreme i deionizirane vode. Skupina B tretirana je slinom nepušača, ostatak postupka isti je kao i za skupinu A. Skupina C tretirana je samo smjesom zubne kreme i deionizirane vode. Skupina D služila je kao kontrola. Alkalno topljivi fluoridi stvoreni na površini cakline određeni su prema metodi Časlavske i suradnika i mjereni ion-selektivnom elektrodom. Koncentracije minerala u slini izmjerene su induktivno spregnutom plazmom sa spektrometrijom masa (ICP-MS). Za analizu podataka korišteni su Friedmanov test i neparametrijski Wilcoxonov test parova.
U slinama pušača i nepušača ne postoji statistički značajna razlika u koncentraciji kalcija, magnezija, fosfata te njihovih omjera kao ni u protoku sline. Omjer kalcija i magnezija u slini nepušača bio je skoro dvostruko veći. Nije dobivena statistički značajna razlika između skupina u količini alkalno topljivih fluorida na površini cakline. Unos fluorida na površinu zubne cakline, uz pastu koja sadrži CPP-ACP kompleks, ne ovisi o mineralnom sastavu sline.

Abstract (english)

The aim of this in vitro study was to assess the effect of smoker's saliva in combination with tooth crèmecontaining calcium and phosphate on the enamel uptake of flouride, assuming that there is a difference in the concentration of calcium in the smoker's and non-smoker's saliva.
Four enamel slabs were cut from each of the ten healthy extracted teeth and arranged into 4 groups (A, B, C, D). Unstimulated saliva samples were donated by twelve male healthy volunteers, six smokers and six non-smokers. The toothcrèmeused in the research was MI Paste Plus (GC, Alsip, Illinois, SAD). Enamel slabs of the group A were treated with smokers' saliva, and then with the tooth crèmeslurry. Group B was treated with non-smokers' saliva, rest of the procedure was the same as with the group A. Group C was treated only with tooth crème slurry. Group D served as a control group. KOH-soluble fluorides were determined by the method of Caslavska et al. and measured by ion selective electrodes. The concentrations of minerals in the saliva were measured by inductive coupled plasma with mass spectrometry (ICP-MS). Friedman's test and Wilcoxon signed-rank test were used to analyse the data.
There is no statistically significant difference in the concentration of calcium, magnesium and phosphate in the saliva of smokers and non-smokers, nor is there any significant difference in their flow of saliva. The ratio of calcium and magnesium in the non-smokers' saliva was twice bigger than in smokers' saliva. There is no statistically significant difference between these groups in the amount of KOH-soluble fluorides on the enamel surface.
The uptake of flourides on the enamel surface, along with a tooth crème which contains CPP-ACP complex does not depend on the mineral composition of saliva.

• Keywords: kalcij; fosfat; fluoridi; slina; pušenje
• Keywords (english): calcium; phosphate; fluorides; saliva; smoking
• Language: croatian
• URN:NBN: urn:nbn:hr:127:897285
• Study programme: Title: dental medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica dentalne medicine (doktor/doktorica dentalne medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-02-15 11:01:48