Utjecaj osobina ličnosti i varijanta gena serotoninskoga sustava na izraženost dentalne anksioznosti

doctoral thesis

• Title (english): Influence of personality traits and serotonergic system polymorphism on the expression of dental anxiety
• Author: Tanja Franćeski
• Mentor: Željko Verzak (mentor)
• Mentor: Dalibor Karlović (komentor)
• Committee member: Silvana Jukić Krmek (predsjednik povjerenstva)
• Committee member: Arijana Lovrenčić-Huzjan (član povjerenstva)
• Committee member: Ana Matošić (član povjerenstva)
• Committee member: Nada Vrkić (član povjerenstva)
• Committee member: Ante Silić (član povjerenstva)
• Granter: University of Zagreb; School of Dental Medicine; (Department of Paediatric and Preventive Dentistry); Zagreb
• Defense date and country: 2018-12-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Dental Medicine
• Universal decimal classification (UDC): 616.31 - Stomatology

Abstract

Budući da je rano bilo uočeno da su lijekovi koji djeluju putem serotoninskoga prijenosnika, djelotvorni u liječenju anksioznih i depresivnih poremećaja, u istraživanjima se polimorfizam gena za serotoninski prijenosnik (5HTTLPR) pokušavao povezati s osobinama ličnosti. Dosadašnja istraživanja bila su usmjerena na povezanost 5-HTTLPR polimorfizma i osobina ličnosti te na povezanost osobina ličnosti i dentalne anksioznosti, ali nije rađeno niti jedno istraživanje o povezanosti 5- -HTTLPR polimorfizma i dentalne anksioznosti.
Ciljevi ovog rada bili su: ispitati povezanost 5-HTTLPR polimorfizma i dentalne anksioznosti, ispitati povezanost 5-HTTLPR polimorfizma i anksioznih dimenzija ličnosti, ispitati povezanost dentalne anksioznosti i anksioznih dimenzija ličnosti, kao i frekvenciju pojedinih alela 5-HTTLPR u ispitivanoj populaciji.
Istraživanje je provedeno na 159 ispitanika, dobrovoljaca, koje je sačinjavalo medicinsko i nemedicinsko osoblje Kliničkog bolničkog centra „Sestre milosrdnice“. U istraživanje su uključeni ispitanici obaju spolova, dobnog raspona od 19 do 59 godina, tjelesno i psihički zdravi. Primijenjeni su upitnici: Corahov upitnik o dentalnoj anksioznosti (eng. Corah Dental Anxiety Scale-Revised, DAS-R), upitnik za mjerenje anksioznosti kao stanja i osobina ličnosti (eng. The State-Trait Anxiety Inventory, STAI) i Eysenckov upitnik ličnosti (eng. The Eysenck Personality Questionnaire, EPQ). Genotipizacija serotoninskog transportera provedena je metodama PCR i RFLP. Sukladno ciljevima, utvrđene su sljedeće frekvencije alela: 55 % L alela i 45 % S alela, odnosno 26,4 % L/L genotipa, 57,2 % L/S genotipa i 16,4 % S/S genotipa. Nađena je pozitivna povezanost S alela i veće izraženosti dentalne anksioznosti. Nije nađena značajna povezanost S alela i anksioznosti kao osobine ličnosti (STAI-O). Utvrđena je pozitivna povezanost S alela s izraženošću neuroticizma te pozitivna povezanost dentalne anksioznosti i anksioznih dimenzija ličnosti (neuroticizma i STAI-O).

Abstract (english)

Background of the research: Since the early observation that serotonin-mediated drugs are effective in the treatment of anxiety and depression disorders, attempts have been made to correlate 5-HTTLPR polymorphism with personality traits. The present research focused on the linkage of 5-HTTLPR polymorphisms and personality traits with the relationship between personality traits and dental anxiety. To date, no studies have been conducted among the Caucasian population on the association of 5-HTTLPR polymorphism and dental anxiety.
Aim of the study: The purpose and aims of this work were to determine the frequency of individual 5-HTTLPR alleles, the association between 5-HTTLPR polymorphism and dental anxiety, the association between 5-HTTLPR polymorphism and anxiety dimensions evaluated by the EPQ and STAI-T Questionnaires, and to explore the association between dental anxiety and anxiety dimensions evaluated by the EPQ and STAI-T Questionnaires.
Methodology: The sample included 159 subjects of both sexes, ranging in age from 19-59 years, of sound physical and mental health (medical and non-medical staff at the Sestre Milosrdnice Clinical Hospital Centre). The DAS-R, EPQ and STAI Questionnaires were applied. Genotypisation of serotonin transporters was conducted using PCR and RFLP methods.
Results: In view of the aims, the allele frequency was established as 55% L allele and 45% S allele. A positive association was found between the S allele and a higher degree of dental anxiety. No statistically significant association was determined between the S allele and anxiety as a personality trait. In addition, a positive association was found between the S allele and neuroticism. A positive association was found between dental anxiety and anxiety as a personality trait (STAI-T), and between dental anxiety and neuroticism.

• Keywords: anksioznost; dentalna anksioznost; ličnost; neuroticizam; serotoninski sustav; 5-HTTLPR polimorfizam
• Keywords (english): anxiety; dental anxiety; personality; neuroticism; serotonin system; 5-HTTLPR polymorphism
• Language: croatian
• URN:NBN: urn:nbn:hr:127:569574
• Study programme: Title: dental medicine; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo, polje dentalna medicina (doktor/doktorica znanosti, područje biomedicine i zdravstvo, polje dentalna medicina)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-01-16 11:52:23