prikaz prve stranice dokumenta Frequency of BRAF Mutations in Dysplastic Nevi, Lentigo Maligna, and Melanoma In Situ
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Scientific paper - Original scientific paper
Frequency of BRAF Mutations in Dysplastic Nevi, Lentigo Maligna, and Melanoma In Situ
Journal of Clinical Medicine, 13 (2024), 16; 4799. https://doi.org/10.3390/jcm13164799
Prkačin, Ivana; Šamija, Ivan; Filipović, Nika; Vucić, Matej; Vučić, Majda; Ferara, Nikola; Šitum, Mirna

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Prkačin, I., Šamija, I., Filipović, N., Vucić, M., Vučić, M., Ferara, N. & Šitum, M. (2024). Frequency of BRAF Mutations in Dysplastic Nevi, Lentigo Maligna, and Melanoma In Situ. Journal of Clinical Medicine, 13. (16). doi: 10.3390/jcm13164799

Prkačin, Ivana, et al. "Frequency of BRAF Mutations in Dysplastic Nevi, Lentigo Maligna, and Melanoma In Situ." Journal of Clinical Medicine, vol. 13, no. 16, 2024. https://doi.org/10.3390/jcm13164799

Prkačin, Ivana, Ivan Šamija, Nika Filipović, Matej Vucić, Majda Vučić, Nikola Ferara and Mirna Šitum. "Frequency of BRAF Mutations in Dysplastic Nevi, Lentigo Maligna, and Melanoma In Situ." Journal of Clinical Medicine 13, no. 16 (2024). https://doi.org/10.3390/jcm13164799

Prkačin, I., et al. (2024) 'Frequency of BRAF Mutations in Dysplastic Nevi, Lentigo Maligna, and Melanoma In Situ', Journal of Clinical Medicine, 13(16). doi: 10.3390/jcm13164799

Prkačin I, Šamija I, Filipović N, Vucić M, Vučić M, Ferara N, and sur.. Frequency of BRAF Mutations in Dysplastic Nevi, Lentigo Maligna, and Melanoma In Situ. Journal of Clinical Medicine [Internet]. 2024 August 15 [cited 2025 April 13];13(16). doi: 10.3390/jcm13164799

I. Prkačin, et al., "Frequency of BRAF Mutations in Dysplastic Nevi, Lentigo Maligna, and Melanoma In Situ", Journal of Clinical Medicine, vol. 13, no. 16, August 2024. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:127:520392. [Accessed: 13 April 2025]

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Metadata
Title (english)Frequency of BRAF Mutations in Dysplastic Nevi, Lentigo Maligna, and Melanoma In Situ
AuthorIvana Prkačin
AuthorIvan Šamija
AuthorNika Filipović
AuthorMatej Vucić
AuthorMajda Vučić
AuthorNikola Ferara
AuthorMirna Šitum
Author's institution(Sestre milosrdnice University Hospital Center)
Author's institutionUniversity of Zagreb
School of Dental Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Background: In melanomas, mutations in the BRAF gene are common and their occurrence represents an early oncogenic event. Our goal was to determine and compare the frequency of BRAF gene mutations in dysplastic nevi (ND) and melanomas in situ (MIS), as well as whether there is a correlation between the presence of BRAF gene mutations and various anamnestic, clinical, and histopathologic variables. Methods: A total of 175 patients—106 with ND, 41 with MIS, and 28 with lentigo maligna (LM) were included in the study. DNA was extracted from tissue samples and analyzed using the competitive allele-specific TaqMan chain reaction by polymerase in real time to detect the presence of BRAF V600E and V600K mutations. The data were compared with anamnestic, clinical, and histopathological data. Results: There is a statistically significant correlation between the presence of BRAF mutation and the diagnosis of melanoma in situ (χ2 test, χ2 = 29.17, p < 0.0001). Patients with LM had a significantly lower incidence of BRAF mutations compared to patients with ND and MIS. There was a significant correlation between the presence of a BRAF mutation and tumor localization, as well as the age of the patient, but no statistically significant correlation between the presence of a BRAF mutation and sex, tumor size, or previous melanoma diagnosis. Conclusions: BRAF mutations in ND are essentially required; however, they are an insufficient oncogenic trigger for the development of melanoma. This research contributes to a better understanding of the etiopathogenesis of melanoma and the role of ND as possible precursor lesions.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of Clinical Medicine
Numberingvol. 13, no. 16, 4799
e-ISSN2077-0383
DOIhttps://doi.org/10.3390/jcm13164799
URN:NBNurn:nbn:hr:127:520392
Publication2024-08-15
Document URLhttps://www.mdpi.com/2077-0383/13/16/4799
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2025-03-06 09:58:20