Određivanje zaostatnih monomera i ostalih gradivnih sastojaka kod debeloslojnih kompozitnih materijala

doctoral thesis

• Title (english): Detection of residual monomers and other leachable components from Bulk Fill resin composite materials
• Author: Matea Lapaš Barišić
• Mentor: Vlatko Pandurić (mentor)
• Committee member: Zrinka Tarle (predsjednik povjerenstva)
• Committee member: Bernard Janković (član povjerenstva)
• Committee member: Dragana Fabris (član povjerenstva)
• Granter: University of Zagreb; School of Dental Medicine; Zagreb
• Defense date and country: 2024-04-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Dental Medicine
• Universal decimal classification (UDC): 616.31 - Stomatology

Abstract

Svrha ovog rada bila je istražiti količinu zaostatnih komponenti (monomera) u krutim i tekućim konvencionalnim (Gradia, Filtek Supreme, Tetric Evo Ceram, Gradia Direct flo, Filtek Supreme flo, Tetric Evo flow) te krutim i tekućim debeloslojnim kompozitnim materijalima (Tetric EvoCeram Bulk fill, X-tra fill, Filtek Bulk Fill, Filtek Bulk Fill flow, SDR, Tetric Evoflow Bulk fill) nakon polimerizacije koji mogu biti odgovorni za alergijska i genotoksična svojstva u usnoj šupljini i organizmu općenito. Šest debeloslojnih (krutih i tekućih) i šest konvencionalnih (krutih i tekućih) kompozitnih materijala je upotrijebljeno u ovoj studiji.
Uzorci svakog od materijala (tri seta od tri uzorka od svakog materijala) su polimerizirani 20 sekundi prema uputama proizvođača i uronjeni u 75% otopinu etanola te pohranjeni na 37 °C u tamni okoliš. Uzorci svakog od materijala analizirani su 24 sata, 7 dana i 28 dana nakon polimerizacije metodom tekućinske kromatografije s dvojnom spektrometrijom masa (LC-MS/MS). Mjerljive količine 2-hidroksiletilmetakrilata (HEMA) su pronađene u oba Gradia materijala i količina nađena unutar različitih vremenskih intervala bila je statistički različita (p< 0,05), s povećanjem otpuštanja tijekom vremena kod materijala Gradia te smanjenjem otpuštanja tijekom vremena kod materijala Gradia Direct flo. Bisfenol-A-glicidil metakrilat (BiS-GMA) nađen je kod Filtek i Tetric materijala. Trietilenglikol-dimetakrilat (TEGDMA) je pronađena u svim materijalima. S druge strane, nije bilo statistički značajne razlike u količini otpuštanja TEGDME kroz različite vremenske periode nakon polimerizacije kod niti jednog od testiranih materijala. Monomeri HEMA, TEGDMA, etilni ester 4 dimetilbenzojeve kiseline (DMA-BEE) i BiS - GMA kod debeloslojnih i konvencionalnih krutih i tekućih kompozitnih materijala mogu biti otpušteni i detektirani nakon polimerizacije. Rezultati ove studije pokazuju da pojedini kompozitni materijali otpuštaju veće količine zaostatnih monomera od drugih.

Abstract (english)

Detection of leachable components from conventional and bulk fill resin composites (high and low viscosity) using liquid chromatography – tandem mass spectrometry (LC-MS/MS) method
Aim: Light cure dental composites are the materials of choice in restorative dentistry due to the their esthetic properties, mechanical strength and applicability in minimally invasive procedures. The time-consuming incremental technique has recently been replaced by a bulk technique thanks to the discovery of bulk – filling composites. Ten percent of the elution of resin composites is caused by free monomers. The aim of this study was to investigate the amount of leachable components (monomers) in high and low viscosity dental bulk-fill resin composites and conventional resin composite
materials after polymerization, because it is indicated that they can be eluted in oral environment and may cause cytotoxic reactions on pulp and living tissues.
Materials and methods: In this study, six commercially bulk fill dental composites were investigated and compared with
six conventional composites. Low and high viscosity composite samples were prepared and light cured according to the standard protocol (20 sec of irradiation with 1200 mW/cm3 in a wavelength range of 380-550 nm) using a LED-curing device, Bluephase G2 and immersed in 75% ethanol solution at 37 °C. After a dark storage period of 24 h, 7 days or 28 days, the eluates were collected and analysed using liquid chromatography coupled with triple quadrupole tandem mass spectrometry (LC-MS/MS).
Results: Detectable amounts of 2-Hydroxyethylmetacrylate (HEMA) were found in both Gradia materials and the amount observed across different time points was statistically different (p<0,05), with the amount in solution increasing for Gradia and decreasing for Gradia Direct flo. Bisphenol A diglycidildimethacrylate (BiS-GMA) was found in Filtek and and Tetric materials. Triethylene glycoldimethacrylate (TEGDMA) was detected in all materials. On the other hand, there were no statistically significant differences in the amounts of TEGDMA detected across different time points in either of the tested materials.
Conclusion: Monomers HEMA, TEGDMA, 4 –dimethylaminobenzoic acid ethyl ester (DMA BEE) and BiS GMA in bulk fill and conventional composites (high and low viscosity) can be eluted into the oral environment after polymerization.
The results indicate that the effect may be ambiguous, as apparently materials from different manufacturers release some monomers more than others.

• Keywords: kompozitne smole; polimerizacija; stomatološki materijali; kromatografija; tekućina
• Keywords (english): Composite Resins; Polymerization; Dental Materials; Chromatography; Liquid
• Language: croatian
• URN:NBN: urn:nbn:hr:127:889179
• Promotion: 2024
• Study programme: Title: dental medicine; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo, polje dentalna medicina (doktor/doktorica znanosti, područje biomedicine i zdravstvo, polje dentalna medicina)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-04-25 08:54:56