Polychromatic Flow Cytometric Analysis of Stromal Vascular Fraction from Lipoaspirate and Microfragmented Counterparts Reveals Sex-Related Immunophenotype Differences

Scientific paper - Original scientific paper

• Author: Lucija Zenic
• Author: Denis Polancec
• Author: Damir Hudetz
• Author: Zeljko Jelec
• Author: Eduard Rod
• Author: Dinko Vidovic
• Author: Mario Staresinic
• Author: Srecko Sabalic
• Author: Trpimir Vrdoljak
• Author: Tadija Petrovic
• Author: Fabijan Cukelj
• Author: Vilim Molnar
• Author: Martin Cemerin
• Author: Vid Matisic
• Author: Petar Brlek
• Author: Zrinka Djukic Koroljevic
• Author: Igor Boric
• Author: Gordan Lauc
• Author: Dragan Primorac
• Author's institution: Josip Juraj Strossmayer University of Osijek
• Author's institution: University of Rijeka
• Author's institution: University of Split
• Author's institution: (Merkur University Hospital)
• Author's institution: (Clinical hospital center Osijek)
• Author's institution: (Sestre milosrdnice University Hospital Center)
• Author's institution: (University Hospital of Split)
• Author's institution: University of Zagreb; School of Medicine
• Author's institution: University of Zagreb; School of Dental Medicine
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences

Abstract (english)

Mesenchymal stem/stromal cells or medicinal signaling cells (MSC)-based therapy holds promise as a beneficial strategy for treating knee OA (osteoarthritis), but there is no standardized protocols nor mechanistic understanding. In order to gain a better insight into the human MSC from adipose tissue applied for autologous OA treatment, we performed extensive comparative immunophenotyping of the stromal vascular fraction from lipoaspirate or microfragmented lipoaspirates by polychromatic flow cytometry and investigated the cellular components considered responsible for cartilage regeneration. We found an enrichment of the regenerative cellular niche of the clinically applied microfragmented stromal vascular fraction. Sex-related differences were observed in the MSC marker expression and the ratio of the progenitor cells from fresh lipoaspirate, which, in female patients, contained a higher expression of CD90 on the three progenitor cell types including pericytes, a higher expression of CD105 and CD146 on CD31highCD34high endothelial progenitors as well as of CD73 on supra-adventitialadipose stromal cells. Some of these MSC-expression differences were present after microfragmentation and indicated a differential phenotype pattern of the applied MSC mixture in female and male patients. Our results provide a better insight into the heterogeneity of the adipose MSC subpopulations serving as OA therapeutics, with an emphasis on interesting differences between women and men.

• Keywords (english): osteoarthritis; mesenchymal stem; stromal cells; stromal vascular fraction; lipoaspirate; microfragmentation; immunophenotyping; endothelial progenitors; pericytes; supra-adventitial-adipose stromal cells
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Genes
• Numbering: vol. 12, no. 12, 1999
• e-ISSN: 2073-4425
• DOI: https://doi.org/10.3390/genes12121999
• URN:NBN: urn:nbn:hr:127:239083
• Publication: 2021-12-16
• Document URL: https://www.mdpi.com/2073-4425/12/12/1999
• Type of resource: Text
• Access conditions: Open access
• Created on: 2022-11-03 12:39:18